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Apolipoprotein E in Healthy Volunteers

Yifen Liu, Handrean Soran, Valentine-Charlton Menys, Michael France, See Kwok, Salam Hama, Rahul Yadav, Basil J Ammor Paul N Durrington

In: Heart UK 26TH ANNUAL CONFERENCE; 27 Jun 2012-29 Jun 2012; 2012.

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Abstract

1Cardiovascular Research Group, University of Manchester, UK 2Cardiovascular Trials Unit, University Department of Medicine, Central Manchester University Hospitals NHS Foundation Trust, Manchester, UK.Background: Apolipoprotein E (apoE) is an important component of plasma lipoproteins and plays a vital role in lipoprotein metabolism. The influence of age and gender on apoE concentration and distribution across lipoproteins is not clear. Methods: ApoE and glycated apoE distributions in different lipoproteins in 32 (18 men and 14 women) healthy volunteers were determined. Results: Total apoE concentration (mean +SEM) was 48.2±2.4 mg/l. Of this 13.3±2.5 mg/l (27.6%) was in VLDL, 10.6±2.2 mg/l (22.0%) in LDL, 14.4±1.2 mg/l (29.9%) in HDL and 9.9± 2.0 mg/l (20.5%) in the density range >1.21g/ml. ApoE concentration in VLDL was strongly correlated with VLDL-apoB and VLDL-TG. There was no significant difference in total apoE between men and women. However, in men VLDL-apoE was significantly higher (15.6±1.4 mg/l vs. 10.3±1.4mg/l, p<0.01) and HDL-apoE was significantly lower (11.7±1.0 vs. 17.9±2.1 p<0.001) compared with women. Total apoE and HDL apoE concentrations increased from the time of the menopause in women, but age had no effect in men. Glycated apoE concentration and the percentage of apoE glycated were highest in VLDL (0.69±0.08mg/l, 1.48%), followed by LDL (0.14±0.03mg/l, 0.29%). Only (0.05±0.006mg/l, 0.10%) apoE was in HDL and (0.02±0.003mg/l, 0.04%) in d>1.21 g/ml were glycated. Conclusions: The apoE concentration and lipoproteins distribution are influenced by age and gender. VLDL and LDL apoE are more susceptible to glycation in vivo.