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- PMID: 22162266
- UKPMCID: 22162266
- DOI: 10.1002/mnfr.201100541
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Molecular and immunological characterization of Mus a 5 allergen from banana fruit.
Aleksic, Ivana; Popovic, Milica; Dimitrijevic, Rajna; Andjelkovic, Uros; Vassilopoulou, Emilia; Sinaniotis, Athanassios; Atanaskovic-Markovic, Marina; Lindner, Buko; Petersen, Arnd; Papadopoulos, Nikolaos G; Gavrovic-Jankulovic, Marija
Molecular nutrition & food research. 2012;56(3):446-53.
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Full-text held externally
- PMID: 22162266
- UKPMCID: 22162266
- DOI: 10.1002/mnfr.201100541
Abstract
SCOPE: Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed. METHODS AND RESULTS: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451±67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. CONCLUSION: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.