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- DOI: 10.1557/opl.2014.517
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Targeting of a magnetic bionanomaterial to HepG2 human hepatocellular carcinoma cells using a galactose terminated lipid.
Booth, A.; Coxon, T.; Gough, J.E.; Webb, S.J
Materials Research Society Symposium Proceedings. 2014;1688.
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Full-text held externally
- DOI: 10.1557/opl.2014.517
Abstract
Magnetic nanoparticle-vesicle aggregates (MNPVs), a controlled release nanostructure, have been enhanced with the inclusion of a novel galactose terminated lipid for cell targeting. Quartz crystal microgravimetry with dissipation (QCM-D) demonstrated that the galactose headgroup was available to bind Erythrina Crista-galli lectin (ECL) when the lipid was incorporated into a lipid bilayer. Similarly, UV-visible spectrophotometry indicated that ECL recognized the galactose headgroup in vesicles, leading to vesicle adhesion and aggregation. Finally, confocal fluorescence microscopy was used to assess the galactose-mediated interaction of both vesicles and MNPVs with HepG2 human hepatocellular carcinoma cells expressing the asialoglycoprotein (ASGPR) galactose receptor.