Related resources
Full-text held externally
- DOI: 10.1038/nature06066
Search for item elsewhere
University researcher(s)
Academic department(s)
An IRF8-binding promoter variant and AIRE control CHRNA1 promiscuous expression in thymus.
Giraud, M, Taubert, R, Vandiedonck, C, Ke, X, LĂ©vi-Strauss, M, Pagani, F, Baralle, F, Eymard, B, Tranchant, C, Gajdos, P, Vincent, A, Willcox, N, Beeson, D, Kyewski, B, Garchon, H
Nature. 2007;448(7156):934-7.
Access to files
Full-text and supplementary files are not available from Manchester eScholar. Full-text is available externally using the following links:
Full-text held externally
- DOI: 10.1038/nature06066
Abstract
Promiscuous expression of tissue-restricted auto-antigens in the thymus imposes T-cell tolerance and provides protection from autoimmune diseases. Promiscuous expression of a set of self-antigens occurs in medullary thymic epithelial cells and is partly controlled by the autoimmune regulator (AIRE), a nuclear protein for which loss-of-function mutations cause the type 1 autoimmune polyendocrine syndrome. However, additional factors must be involved in the regulation of this promiscuous expression. Here we describe a mechanism controlling thymic transcription of a prototypic tissue-restricted human auto-antigen gene, CHRNA1. This gene encodes the alpha-subunit of the muscle acetylcholine receptor, which is the main target of pathogenic auto-antibodies in autoimmune myasthenia gravis. On re-sequencing the CHRNA1 gene, we identified a functional bi-allelic variant in the promoter that is associated with early onset of disease in two independent human populations (France and United Kingdom). We show that this variant prevents binding of interferon regulatory factor 8 (IRF8) and abrogates CHRNA1 promoter activity in thymic epithelial cells in vitro. Notably, both the CHRNA1 promoter variant and AIRE modulate CHRNA1 messenger RNA levels in human medullary thymic epithelial cells ex vivo and also in a transactivation assay. These findings reveal a critical function of AIRE and the interferon signalling pathway in regulating quantitative expression of this auto-antigen in the thymus, suggesting that together they set the threshold for self-tolerance versus autoimmunity.
Keyword(s)
Age of Onset; Alleles; Cell Line; Gene Expression Regulation; Humans; Protein Binding; cytology: Thymus Gland; epidemiology: France; epidemiology: Great Britain; epidemiology: Myasthenia Gravis; genetics: Polymorphism, Single Nucleotide; genetics: Promoter Regions, Genetic; genetics: RNA, Messenger; genetics: Receptors, Nicotinic; genetics: Transcription Factors; genetics: Transcription, Genetic; metabolism: Epithelial Cells; metabolism: Interferon Regulatory Factors