Dis Markers. 2005;21( 2):75-7.
Increased expression of epithelial cell-derived neutrophil-activating peptide (ENA-78)
has been reported in several immune and inflammatory conditions suggesting its role
in inflammatory response. We have identified two single nucleotide polymorphisms in
the promoter and exon 2 of the ENA-78 gene by scanning the full length gene using
DHPLC DNA fragment analysis and DNA sequencing. The polymorphism at position +398
(A/G from the first ATG codon) in exon 2 results in a synonymous substitution not
resulting in an amino acid change. The promoter polymorphism was found at position
-156 (C/G from the first ATG codon). An assay was designed for the detection of the
polymorphisms using SNapshot ddNTP primer extension, followed by capillary electrophoresis
(ABI 3100). Allele and genotype frequencies for the promoter -156 polymorphism are
presented for 107 healthy Spanish and 54 UK Caucasians. Frequencies for the exon 2
polymorphism are also presented for 63 UK Caucasians.